Senior Protein Engineer

Key Responsibilities include:

• Conceptualize, design, construct, and implement methodologies for novel enzyme and protein discovery

• Periodically research literature to focus and supplement research on metabolic and signaling interventions for mammalian & non-mammalian animal cells

• Directly support proteomics work by providing expertise on design, post-translational modifications and testing of proteins and protein analogs

• Improve kinetic properties of enzymes

• Increase stability and biological activity of proteins

• Perform techniques such as SDS-PAGE,

• Perform assessments for protein engineering method selection including site-directed mutagenesis, DNA shuffling & chemical modification on a case-by-case basis

• Perform mechanistic characterization of protein activity and stability

• Maintain up-to-date expertise in the field across technologies relevant to the role

• Independently advance projects through collaboration with a diverse team of wet-lab scientists, engineers, and computational scientists

• Test the performance of the engineered strains using growth, metabolic, and productivity assays including HPLC, ELISA, SDS-PAGE, Western Blotting, etc.

• Collaborate cross-functionally to plan and execute demanding cell engineering and screening campaigns that produce strains to meet bioprocess needs and GMP requirements

• Interpret and present experimental data to scientific, engineering, and leadership teams

• Design and implement screening or selection methods to quickly validate new constructs

• Utilize bioinformatics DNA sequence analyses to design and validate successfully engineered strains

• Conceptualize, design, construct, and implement platforms for engineering of the proteins e.g, multi-specificity, stability, conformational restraint, effector function, expression and framework optimization

• Keep abreast of scientific literature in the field and implement novel modalities and protocols.

• Provide expertise in evaluation of new platform technologies.

• Proactively search for novel technologies or product opportunities and establish collaborations and research programs to deliver innovative technical solutions.

• Design and construct cell strains via optimization of proteins in cell-free environments based on understanding of cell metabolism, review of relevant literature, and collaboration with scientific leadership.

The ideal candidate will possess the following qualities & qualifications:

• Doctoral degree (PhD or equivalent) in a related scientific discipline + 4 years of research experience or MS degree in related field + 10 years of research experience

• Experience with rational or screen-based engineering to optimize protein activity

• Experience with cell-based assays

• Track record of moving projects forward

• Experience with CRISPR-Cas and/or Antibody engineering, expression, purification, and characterization

• Experience with chemical and/or bioconjugation methods and related analytical techniques

• Experience in a variety of chromatography techniques including affinity, IEX, and SEC

• Experience or a high level of familiarity with NGS-based analytical techniques

• Experience or a high level of familiarity with genome editing

• Experience or a high level of familiarity with animal metabolism and signaling pathways

• Effective oral and written communications skills.

• High personal ethical and quality standards.

• Exceptional problem solving and decision-making skills.

• Must be able to work productively in an interdisciplinary team environment

• Eagerness to independently learn new software tools and laboratory techniques