Scientist I (Gene Therapy/ Cell Cloning)

This role involves evaluating novel mRNA and DNA designs using a range ofmolecular biology techniques, including plasmid cloning, in vitro validation,and the production of in vitro-transcribed mRNA. The Scientist I will bea vital contributor to our non-viral cell and gene therapy delivery platform,primarily focused on R&D for innovative nucleic acid payload technologies.

Our client, a worldleader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA.

Job Duration: LongTerm Contract (Possibility Of Extension)

Pay Rate : $46.43/hr onW2

CompanyBenefits: Medical, Dental, Vision, Paid Sick leave, 401K

The Scientist I will bea vital contributor to our non-viral cell and gene therapy delivery platform,primarily focused on R&D for innovative nucleic acid payload technologies.This role involves evaluating novel mRNA and DNA designs using a range ofmolecular biology techniques, including plasmid cloning, in vitro validation,and the production of in vitro-transcribed mRNA. To verify successfulexpression, the scientist will utilize assays such as Western blot, qRT-PCR,and luciferase reporter assays.

Job Responsibilities:

·Design and cloneexpression vectors and IVT donor plasmids to support research initiatives.

·Execute DNA and mRNAtransfections in mouse and human cell lines.

·Assess nucleic acidexpression through molecular methods like qPCR, reporter assays and Westernblotting.

·Maintenance of detailedelectronic laboratory notebooks (ELN) and preparation of summary slides

Required Qualifications:

• Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
• MolecularBiology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
• FunctionalAssays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
• CellCulture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g.,T cells, monocytes, hepatocytes).
• Desired proficiency in Snapgene, Prism, and MS Office.

If interested, pleasesend us your updated resume at

hr@dawarconsulting.com

/

akansha@dawarconsulting.com