Our client, a world leader in life sciences and biotechnology, islooking for a “Scientist – Discovery Biology/ T cells” based in SanDiego, CA.
Job Duration: Long Term Contract (Possibility of Extension)
Pay Rate: $49.20/hr on W2
Company Benefits: Medical, Paid Sick leave,401K
Join the Discovery Biology team to support a high-priority binderscreening campaign for T-cell-based therapies. Characterize candidate binders(scFvs/VHHs) for potency, specificity, affinity, functionality, and safetythrough T-cell engineering and functional validation assays.
Key Responsibilities
- Perform non-viral engineering of primary human T cells using mRNA or transposon-based DNA systems via electroporation/nucleofection; monitor cell viability, recovery, and synthetic receptor expression.
- Isolate, maintain, and expand primary T cells from PBMCs and Jurkat cell lines using aseptic techniques.
- Execute high-throughput binder screening and multi-color flow cytometry (FACS) to assess surface expression and phenotypic markers; analyze data usingFlowJo.
- Conduct T-cell activation and functional assays, including cytotoxicity assays (IncuCyte live-cell imaging/LDH release), cytokine profiling (ELISA/MSD/Luminex), and proliferation assays (CFSE).
- Record protocols, deviations, raw data, and experimental results accurately in an ElectronicLab Notebook (ELN) to support data integrity and regulatory filings.
- Support screening scale-up using liquid-handling robotics, as applicable.
Required Qualifications
- Bachelor's orMaster's degree in Immunology, Molecular Biology, Bioengineering, or a related biological science.
- Minimum 2 years of hands-on wet-lab experience, preferably in an industry setting.
- Strong aseptic technique and experience isolating primary T cells from PBMCs.
- Mandatory expertise in flow cytometry acquisition and data analysis.
Preferred Qualifications
- Hands-on experience with non-viral T-cell transfection/electroporation or nucleofection.
- Experience with liquid-handling robotics and high-throughput screening workflows.
- Proficiency inFlowJo and functional T-cell assay techniques.
If interested, please send us your updated resume at
hr@dawarconsulting.com
/
akansha@dawarconsulting.com