Our client, a world leader in life sciences and biotechnology, is
looking for a “Scientist – Discovery Biology/ T cells” based in San
Diego, CA.
Job Duration: Long Term Contract (Possibility of Extension)
Pay Rate: $49.20/hr on W2
Company Benefits: Medical, Paid Sick leave,
401K
Join the Discovery Biology team to support a high-priority binder
screening campaign for T-cell-based therapies. Characterize candidate binders
(scFvs/VHHs) for potency, specificity, affinity, functionality, and safety
through T-cell engineering and functional validation assays.
Key Responsibilities
- Perform non-viral
engineering of primary human T cells using mRNA or transposon-based DNA
systems via electroporation/nucleofection; monitor cell viability,
recovery, and synthetic receptor expression.
- Isolate, maintain,
and expand primary T cells from PBMCs and Jurkat cell lines using aseptic
techniques.
- Execute
high-throughput binder screening and multi-color flow cytometry (FACS) to
assess surface expression and phenotypic markers; analyze data using
FlowJo.
- Conduct T-cell
activation and functional assays, including cytotoxicity assays (IncuCyte
live-cell imaging/LDH release), cytokine profiling (ELISA/MSD/Luminex),
and proliferation assays (CFSE).
- Record protocols,
deviations, raw data, and experimental results accurately in an Electronic
Lab Notebook (ELN) to support data integrity and regulatory filings.
- Support screening
scale-up using liquid-handling robotics, as applicable.
Required Qualifications
- Bachelor's or
Master's degree in Immunology, Molecular Biology, Bioengineering, or a
related biological science.
- Minimum 2 years of
hands-on wet-lab experience, preferably in an industry setting.
- Strong aseptic
technique and experience isolating primary T cells from PBMCs.
- Mandatory
expertise in flow cytometry acquisition and data analysis.
Preferred Qualifications
- Hands-on
experience with non-viral T-cell transfection/electroporation or
nucleofection.
- Experience with
liquid-handling robotics and high-throughput screening workflows.
- Proficiency in
FlowJo and functional T-cell assay techniques.
If interested, please send us your updated resume at
hr@dawarconsulting.com
/
akansha@dawarconsulting.com