Perform complex Cell Biology, Molecular Biology, and Biochemistry techniques with minimal supervision including but not limited to:
- Mammalian cell culture
- Cell biological assays
- DNA/RNA isolation
- Molecular cloning
- qPCR
- Western blotting
- Protein extraction and purification
- Immunofluorescence microscopy
Maintain mouse colonies which includes:
- Breeding and weaning
- Genotyping
- Harvesting tissues
- Performing physiological assessments
Protocol Design and Refinements
- With minimal supervisor guidance, refines, adapts, and makes decisions to switch experiment parameters in response to situational demands of experiments.
- Assists in preparing experimental designs, technical protocols and techniques, and Standard Operating Procedures (SOPs).
Record-keeping, Analysis, and Presentations
- Communicates effectively all aspects of experiments with PI or other laboratory staff.
- Keeps accurate and detailed records of experiments, results, and enters data in laboratory databases.
- Catalogs specimens and samples.
- Performs independent analysis and interpretation of data for investigator .
Operational Support
- Orders and catalogs laboratory supplies and supports laboratory operations.
- Operating, maintaining and troubleshoot highly specialized and sophisticated scientific apparatuses, equipment, and instruments.
Knowledge, Skills, and Abilities:
- Aptitude for quick learning and multi-tasking.
- Knowledge of cell biology, molecular biology, and biochemistry principles and techniques.
- Expertise in cell culture, molecular cloning, nucleic acid analysis, protein biochemistry, and microscopy techniques.
- Skill in troubleshooting laboratory procedures and optimizing experimental protocols.
- Experience handling laboratory mice.
- Strong organizational, communication, and project management skills.
- Ability to work independently while contributing effectively within a collaborative research team.