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Laboratory Technician II

University of Florida

  • Gainesville, FL
  • 2 days ago
  • $19.23–$22 Per Hour

Highlights

Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines; immunoassays to gene therapy vectors and transgenes, including ELISAs. The Laboratory Technician II position described herein performs research in the University of Florida Pediatric Cancer Immunotherapy Initiative (PCI2), with a focus on work being conducted in the laboratory of Dr. Leighton Elliott, Department of Medicine, Division of Hematology & Oncology.

Numbers & Facts

LocationGainesville, FL
Salary$19.23–$22 Per Hour

Description

The Laboratory Technician II position described herein performs research in the University of Florida Pediatric Cancer Immunotherapy Initiative (PCI2), with a focus on work being conducted in the laboratory of Dr. Leighton Elliott, Department of Medicine, Division of Hematology & Oncology. The incumbent will perform laboratory experiments using gene therapy applications and procedures established in this lab. The individual in this position is expected to function under general supervision and troubleshoot simple to moderately complex scientific issues independently (minimally alter or modify procedures if necessary). The position requires work with rodent animal models, histological work, and molecular/cellular work.
Classification Title:

Laboratory Technician II

Classification Minimum Requirements:

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Job Description:

Animal Studies: Participating in treating animals with pharmacological agents or immunotherapy either through i.p., i.m., i.v., or intracranial injection. Other duties with the mice include assisting with MRI, CT, and MPI imaging techniques. Assist with experimental approaches using magnetic nanoparticles. Plays a key role in regulatory management (IACUCs).

Histological Work/Analysis: Collecting organs and other tissues and preparing for histopathology following the preparation of slides, staining with standard histology stains such as H&E, as well as with immunohistochemistry and immunofluorescence will be done. Conduct image capture with fluorescent microscope, confocal microscope, multiphoton confocal microscope and analysis of tissue following intervention studies.

Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines; immunoassays to gene therapy vectors and transgenes, including ELISAs. This may include expansion/maintenance of cell lines and analysis of transfection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots. Perform multiparameter flow cytometry and develop proficiency setting multicolor flow panels.

Regulatory Compliance: Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met. Train new laboratory members to ensure compliance standards are met.

Training: Train and supervise graduate and undergraduate students performing diagnostic procedures related to immune therapy experiments.

Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include performing tasks and experiments as directed by the Principal Investigator

Expected Salary:

$19.23 - $22.00/hour

Required Qualifications:

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Preferred:
  • Animal colony management and experience handling rodents (highly preferred).
  • Experience administering treatments i.v., i.p., i.m., and/or intracranially to rodents (highly preferred).
  • Experience maintaining cell lines, performing plasmid transfections/viral infections, immunohistochemistry, FACS, rt-PCR, genotyping, immunohistochemistry, western blotting, microscopy
  • Advanced degree in related scientific field.
  • Working knowledge of basic laboratory techniques.
  • Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.
  • Strong interest in translational cancer research
Special Instructions to Applicants:

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

This requisition has been reposted. Previous applicants are still under consideration and need not apply.

Health Assessment Required:Yes

 

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