Scientist I/II, In Vitro Pharmacology

The Team

Alloy’s In Vitro Pharmacology team collaborates with drug discovery partners to conduct in vitro studies on therapeutic candidates across diverse modalities. Our team delivers comprehensive functional screening and characterization of biologics to support discovery and selection of lead candidates. Primary modalities are monoclonal, multispecific, and TCR-mimic antibodies, but also include cell therapies and genetic medicines. As Alloy Tx enters its next phase of growth, we are expanding our capabilities to include mechanistic studies that enhance preclinical development and further advance our translational research efforts.

The Role 

We are seeking a highly motivated Scientist for in vitro screening and pre-clinical development to join our dynamic and fast-paced team. In this role, you will support the execution of in vitro functional cell-based assays to evaluate the potency, mechanism of action, and pharmacological properties of biologic therapeutics. This role requires exceptional organizational skills, a commitment to scientific excellence, and the ability to adapt to a dynamic environment. You will report to the Associate Director, In Vitro Pharmacology and will work collaboratively in a matrixed environment to drive biologics discovery while supporting rapid decision-making in a fast-moving biotech setting.

How You’ll Drive Impact 

• Execute: Support in vitro functional characterization of drug candidate molecules.
• Develop: Bring a proactive mindset to optimize workflows for more efficient and effective data generation.
• Deliver: Support screening campaigns to meet experimental timelines for monoclonal and multispecific antibodies, as well as TCR-mimic antibodies.
• Collaborate: Work closely across multi-disciplinary teams in biologics discovery programs spanning all Alloy sites. Engage with an inclusive and collaborative work environment.
• Communicate: Clearly convey scientific concepts, experimental results, and interpretations to internal and external stakeholders. 

Principal Responsibilities 

• Develop and optimize cell-based activity assays for primary and/or engineered human immune cells (T cells, NK cells, macrophages, etc)
• Process human leukopaks, isolate and characterize human PBMCs.
• Expansion and analysis of primary or genetically modified primary T cells and other immune cells
• Utilize an array of analytical and molecular techniques to investigate the dynamics and responses of T cells and other immune cells to antibody-based therapeutics
• Execute cell-based functional assays using high-throughput approaches in primary cells, immortalized reporter cell lines, and other disease-relevant cellular systems.
• Contribute to study design, execution, and data analysis.
• Support day-to-day lab and experimental workflows.
• Effectively collaborate across divisional or departmental teams and with external partners to drive projects forward and obtain test materials and reagents.
• Present experimental data and findings to cross-functional teams and relevant stakeholders.
• Ensure timely and successful execution of project activities in alignment with project plans and priorities.
• Maintain detailed and accurate laboratory records in compliance with industry standards.
• Maintain laboratory organization and ensure compliance with safety protocols.

Qualifications

• PhD in Immunology, Cell or Molecular Biology, or a related field, with 0-2 years (Scientist I) or 3-5 years (Scientist II) of relevant industry experience. 
• Strong background in T cell biology research and functional evaluation in an academic or industry setting.
• Experienced in the development and execution of in vitro assays to characterize T cell activation and cytotoxicity (e.g. cell killing, cytokine release, proliferation, etc.) using both primary or genetically modified cell models.
• Flow cytometry and fundamental cell and molecular biology techniques required.
• Significant cell culture experience with primary and immortalized cell culture – isolation, handling, expansion and characterization of primary human immune cells.
• Experience handling human blood, leukopak processing, and PBMC isolations.
• Experience working with antibody-based therapeutics preferred.
• Direct experience with cell line engineering (e.g. CRISPR, lentiviral, etc) preferred.
• Experience with live cell imaging (e.g. Incucyte) a plus.
• Excellent organization, clear communication and teamwork abilities in a multidisciplinary setting. 
• Thrives working on a team and collaborating with others to achieve project goals.
• Enjoys a dynamic and exciting work environment. 
• Detail oriented, with rigorous scientific thinking and a passion for drug discovery to improve patients’ lives.

• Competitive base and equity compensation commensurate with level of experience and independence
• 401(k) company match

• Generous personal and family medical, dental and vision benefits with 100% of premiums and deductibles covered
• Company-paid disability (STD, LTD) and life insurance
• Paid parental leave
• Family planning support up to $45,000 (e.g., IVF/PGT, adoption, surrogacy, egg retrieval)

• Unlimited PTO (paid time off) and flexible schedules
• Annual stipend for continuing education with commitment to your career through individualized professional development plan
• Wellness and Extensive Employee Assistance Program (EAP) including resources for mental wellness

Pay Transparency
At Alloy Therapeutics, we believe in fostering trust and open communication. For this role, the estimated annual base salary range is $126,000 - $144,000, with the final offer based on factors like your experience, skills, and alignment with our needs.

Additionally, this role is eligible for equity compensation, reflecting our commitment to shared success as we work together to make better medicines.

We are proud to offer competitive compensation and benefits, aiming to support our team’s professional and personal well-being. If you have any questions about pay or benefits, we’re here to help.

recruiting@alloytx.com

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