Cell Line Development Research Associate

Our client, a world leader in diagnostics and life sciences, is looking for a "Cell Line Development Research Associate<\/b>” based out of South San Francisco, CA.<\/b><\/span>
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Job Duration: Long term Contract (Possibility Of Further Extension)<\/span><\/b>
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Pay Rate: $42/hr on W2<\/span><\/b>
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Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K<\/span><\/b>
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Cell Line Development Research Associate is involved in the creation of stable cell lines for the recombinant adeno -associated viral vector (rAAV) production processes . He/she executes experiments, produces relevant documentation, and works under supervision. <\/span>
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Responsibilities:<\/span><\/u><\/b>
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• Culturing mammalian cells for viral production in shake flasks, spinner flasks, spin tubes, deep well and automation platforms.<\/span>
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• Performing experiments in the development, optimization and scale -up of AAV producer cell lines.<\/span>
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• Preparing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.<\/span>
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• Optimization of transfection and transduction protocols for newly engineered cell lines.<\/span>
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• Maintaining an accurate laboratory notebook.<\/span>
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• Ensuring work conforms to applicable quality and safety standards.<\/span>
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  Qualifications:<\/span><\/u><\/b>
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  • B.S. with 3 -6 years of experience, MSc with 2 -5 years of relevant scientific or technical experience in the life sciences industry or equivalent<\/span>
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  • Experience in cell culture including materials, instruments and technologies is essential.<\/span>
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  • Experience with viral vector production is preferred.<\/span>
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  • Hands -on experience in mammalian cell culture production processes is a critical requirement. This includes small scale (shaker flasks, spinner flasks, spin tubes, deep well) and/or bench top bioreactor scale.<\/span>
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  • Experience with creation, analysis and characterization of new engineered cell lines and stability studies (sub cloning, clonal expansion, transient transfection and expression screening) is a strong plus.<\/span>
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  • Experience in molecular biology techniques (plasmid design, molecular cloning, SDS<\/span>
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  • PAGE/western blotting, qPCR, etc) is a strong plus.<\/span>
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  • Automation and ELN experience is a strong plus.<\/span>
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  • Experience in laboratory automation hardware and software is a plus.<\/span>
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  • Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule.<\/span>
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  • Handling large volumes of cell culture or related materials, such as tens of liters, may be required. Also, several viral materials, including AAV may be involved in the work.<\/span>
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    If interested, please send us your updated resume at<\/span>
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    hr@dawarconsulting.com

    akansha@dawarconsulting.com

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