MEDICAL & MOLECULAR GENETICS (IN-MMGE-IUINA) Job Summary
This position will operate under the Biorepository Operations Manager to perform advanced laboratory services related to specialized specimen processing and assist in the design and implementation of new protocols. This position will assist in the development of technical SOPs and creating work flow sheets for protocol specific processing. They will train new staff members and hourly employees in designated sample processes. They will support implementation of new SOPs and comply with all established SOPs. Actively participate in SOP revisions in coordination with the QA Specialist and the Biorepository Operations Manager.
• Working with PIs and study coordinators to determine specific protocol requirements from multiple studies (internal and external collaborators). Maintenance and monitoring of critical facility equipment used in the sample processing. Assisting in new equipment validations and startups. Generating data critical to final equipment reports.
• Perform advanced and specialized laboratory procedures in the molecular biology and cell culture laboratory. Procedures include, but are not limited to, creation, immortalization and cryopreservation of lymphoblastoid and fibroblast cell lines. Isolation of peripheral blood mononuclear cells and extraction of DNA from tissue, whole blood, buffy coats, cell pellets and saliva.
• E xtraction of total RNA and miRNA from whole blood. Spectrophotometric analysis of DNA and RNA samples. Gel electrophoresis and separation of whole blood components in the stat lab. Required to make reagents for samples processing and conduct QC on reagents. Expected to design, modify and maintain appropriate documentation of processing activities.
• Receive international and local sample shipments. Accession samples into Oncore Database and other required systems. Maintain chain of custody tracking thru sample storage locations while processing or in permanent storage locations. Pull samples from locations to prepare for sample distributions. Standardize DNA for shipments using robotic handling equipment (i.e. Tecan). Sub-aliquot as requested by funder. Trained in IATA shipping requirements. Ship samples locally and internationally. Required Qualifications Bachelor's degree (BS/BA) in biology/ chemistry/ biotechnology or equivalent and one year of laboratory experience; OR master's degree (BS/BA) in biology/ chemistry/ biotechnology or equivalent and six months of laboratory experience.
Ability to use scientific rules for laboratory directives and for troubleshooting. Ability to perform routine maintenance of laboratory equipment. Strong communication skills. Ability to prioritize and manage time. Able to work on multiple tasks and work well as part of a team. Working Conditions / Demands
Must be able to perform the essential functions of the job with or without an accommodation.
Equipment utilized: Centrifuges, pipetting devices, vortex, incubators, -80 freezers, LN2 tanks, controlled rate freezers, biologic safety cabinets, and personal computer. Work Location
Indianapolis, Indiana Job Classification
Salary Plan: TE
Salary Grade: 07
Job Function: Research Posting Disclaimer
This posting is scheduled to close at 11:59pm EST on the advertised Close Date. This posting may be closed at any time at the discretion of the University, but it will remain open for a minimum of 5 business days. To guarantee full consideration, please submit your application within 5 business days of the Posted Date. Equal Employment Opportunity
Indiana University is an equal employment and affirmative action employer and a provider of ADA services. All qualified applicants will receive consideration for employment without regard to age, ethnicity, color, race, religion, sex, sexual orientation, gender identity or expression, genetic information, marital status, national origin, disability status or protected veteran status. Indiana University does not discriminate on the basis of sex in its educational programs and activities, including employment and admission, as required by Title IX. Questions or complaints regarding Title IX may be referred to the U.S. Department of Education Office for Civil Rights or the university Title IX Coordinator. URL blocked - click to apply
Ribonucleic Acid Sequencing