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  • Greenfield, IN 46140

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Microbiologist - Cell Culture

Pace Analytical • Greenfield, IN

Posted 1 month ago

Job Snapshot

Full-Time
Degree - 4 Year Degree
Other Great Industries
Science

Job Description

Overview

The successful candidate will manage the day-to-day maintenance of a panel of traditional continuous mammalian cell lines. They will also support efforts to create and utilize cell lines that have been modified to express a protein.

Responsibilities
  • Perform routine mammalian cell culture maintenance (break-out, split/passage/expand, freeze down) for both adherent and suspension cell lines
  • Utilize manual and automated cell and viability counters and automated bioanalyzers e.g., Nova instrumentation
  • Maintain cells in flatware, shaker flasks, and lab-scale bioreactors
  • Receive and fill orders from scientists to provide cells in a variety of formats: plates, flasks, or spinner cultures
  • Ensure Elanco cell line inventory is constantly up-to-date and cell lines are within passage limits
  • Generate research cell banks (RCBs)
  • Strictly adhere to sterile technique and maintain endotoxin free laboratory materials.
  • Transiently transfect suspension HEK-293 or CHO cell cultures for protein expression studies
  • Create stable, recombinant HEK-293 and CHO pools
  • Clone cells by limited dilution or FACs
  • Perform protein expression studies including scale-up, feed addition, monitoring of viability and productivity (incl. by SDS-PAGE, capillary electrophoresis, or BLI (Octet)), harvest, and clarification

Job Requirements

  • Bachelor's degree in Microbiology, Biochemistry, Cell Biology, or related science
  • Experience maintaining mammalian cell cultures
  • Experience with expressing proteins
  • Strong communication skills
OFCCP Statement Pace Analytical is an Equal Opportunity Employer and will not discriminate against any applicant for employment on the basis of race, age, religion, sex, veterans, individuals with disabilities, sexual orientation, or gender identity.
Job ID: 2019-3161
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